货号：0731-2 Rex1 (Zfp-42) 是多能性的已知标志物，通常存在于未分化的胚胎干细胞中。除了作为多能性标志物外，其调控对于维持多能状态也至关重要。随着细胞开始分化，Rex1 会急剧下调。在胚胎发生过程中，内细胞团 (ICM) 与滋养层分离。研究发现，源自 ICM 和滋养外胚层的干细胞表达高水平的 Oct3/4 和 Rex1。随着 ICM 成熟并开始形成外胚层和原始外胚层，ICM 中的细胞被发现是异质性群体，Rex1 表达水平不同。Rex1−/Oct3/4− 触发滋养外胚层分化，而 Rex1+/Oct3/4+ 细胞主要分化为原始内胚层和中胚层。此外，Rex1−/Oct3/4+ 细胞分化为原始外胚层细胞，即体细胞谱系。
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| 产品名称 | ZFP-42/REX-1 Rabbit Polyclonal Antibody |
| ZFP-42/REX-1 兔多克隆抗体 | |
| 抗体类型 | Rabbit Polyclonal Antibody |
| 兔多克隆抗体 | |
| 免疫原 | Synthetic peptide within Human ZFP-42 / REX-1 aa 261-310 / 310 |
| 人 ZFP-42 / REX-1 氨基酸 261-310 / 310 的合成肽 | |
| 种属反应性 | Human |
| 人类 | |
| 验证应用 | WB |
| 蛋白质印迹 (WB) | |
| 分子量 | Predicted band size: 35 kDa |
| 预测条带大小：35 kDa | |
| 阳性对照 | D3 |
| D3 | |
| 偶联 | Unconjugated |
| 非偶联 | |
| RRID | AB_3068647 |
| AB_3068647 | |
| - 形态 | Liquid |
| 液体 | |
| - 浓度 | 1ug/ul |
| 1ug/ul | |
| - 存放说明 | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| 解冻后储存于 +4℃。分装后储存于 -20℃ 或 -80℃。避免反复冻融。 | |
| - 存储缓冲液 | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| 1*PBS (pH7.4), 0.2% BSA, 40% 甘油。防腐剂：0.05% 叠氮化钠。 | |
| - 亚型 | IgG |
| IgG | |
| - 纯化方式 | Immunogen affinity purified |
| 免疫原亲和纯化 | |
| - WB | 1:5,000 |
| 1:5,000 | |
| 靶点 | |
| - 功能 | Rex1 (Zfp-42) is a known marker of pluripotency, and is usually found in undifferentiated embryonic stem cells. In addition to being a marker for pluripotency, its regulation is also critical in maintaining a pluripotent state. As the cells begin to differentiate, Rex1 is severely and abruptly downregulated. During embryogenesis, the inner cell mass (ICM) is separated from the trophoblast. The stem cells derived from the ICM and trophectoderm have been found to express high levels of Oct3/4 and Rex1. As the ICM matures and begins to form the epiblast, and primitive ectoderm, the cells in the ICM have been found to be a heterogenous population, with varying levels of Rex1 expression. Rex1−/Oct3/4− triggers trophectoderm differentiation, while Rex1+/Oct3/4+ cells predominantly differentiate into primitive endoderm and mesoderm. Also, Rex1−/Oct3/4+ cells differentiate into cells of primitive ectoderm, the somatic cell lineage. Studies have shown that PEG3 and Nespas are downstream targets of Rex1. Rex1 can control the expression of Peg3 via epigenetic changes. YY1 has been shown to be involved in setting up DNA methylation on the maternal allele of PEG3 during oogenesis. Rex1 was found to protect the paternal allele from being methylated, and keep the PEG3 gene unmethylated during early embryogenesis. Rex1 exhibits gene control in developing embryos via its epigenetic control on genes such as PEG3, which has been identified as playing a key role in fetal growth rates. The only adult tissue Rex1 has been identified in are the testicles. Using in situ hybridization it was determined that the spermatocytes in the more inner layers of the testicles are expressing Rex1. Thus, the male germ cells undergoing meiosis are the specific cells in the testicles that express Rex1. It has not been observed, however, that Rex1 is expressed in the female germ cells. |
| Rex1 (Zfp-42) 是多能性的已知标志物，通常存在于未分化的胚胎干细胞中。除了作为多能性标志物外，其调控对于维持多能状态也至关重要。随着细胞开始分化，Rex1 会急剧下调。在胚胎发生过程中，内细胞团 (ICM) 与滋养层分离。研究发现，源自 ICM 和滋养外胚层的干细胞表达高水平的 Oct3/4 和 Rex1。随着 ICM 成熟并开始形成外胚层和原始外胚层，ICM 中的细胞被发现是异质性群体，Rex1 表达水平不同。Rex1−/Oct3/4− 触发滋养外胚层分化，而 Rex1+/Oct3/4+ 细胞主要分化为原始内胚层和中胚层。此外，Rex1−/Oct3/4+ 细胞分化为原始外胚层细胞，即体细胞谱系。研究表明，PEG3 和 Nespas 是 Rex1 的下游靶标。Rex1 可以通过表观遗传变化控制 Peg3 的表达。YY1 已被证明在卵子发生过程中参与 PEG3 母源等位基因的 DNA 甲基化。研究发现，Rex1 可以保护父源等位基因不被甲基化，并在早期胚胎发生过程中保持 PEG3 基因未甲基化。Rex1 通过对 PEG3 等基因的表观遗传控制在发育中的胚胎中表现出基因调控作用，PEG3 已被确定在胎儿生长速率中起关键作用。Rex1 唯一在成人组织中被发现的是睾丸。通过原位杂交确定，睾丸内层的精母细胞表达 Rex1。因此，进行减数分裂的雄性生殖细胞是睾丸中表达 Rex1 的特定细胞。然而，尚未观察到 Rex1 在雌性生殖细胞中表达。 | |
| 背景文献 | 1. Mongan N.P., et al. Mol. Carcinog. 45:887-900(2006) 2. Raman J.D., et al. Carcinogenesis 27:499-507(2006) |
| 1. Mongan N.P., 等. Mol. Carcinog. 45:887-900(2006) 2. Raman J.D., 等. Carcinogenesis 27:499-507(2006) | |
| 序列相似性 | Belongs to the krueppel C2H2-type zinc-finger protein family. |
| 属于 krueppel C2H2 型锌指蛋白家族。 | |
| 组织特异性 | Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines. |
| 在肾脏、表皮角质形成细胞、前列腺上皮细胞、支气管和小气道肺上皮细胞中表达（蛋白质水平）。在恶性肾脏和多种癌细胞系中表达（蛋白质水平）。在胚胎干细胞、肾脏、表皮角质形成细胞、前列腺上皮细胞、支气管和小气道肺上皮细胞中表达。在胚胎癌、精原细胞瘤、恶性肾脏和多种癌细胞系中表达。 | |
| 翻译后修饰 | Polyubiquitinated by RNF12, leading to proteasomal degradation. |
| 被 RNF12 多泛素化，导致蛋白酶体降解。 | |
| 亚细胞定位 | Nucleus |
| 细胞核 | |
| UNIPROT # | SwissProt: Q96MM3 Human |
| SwissProt: Q96MM3 人类 | |
| 别名 | hREX-1 antibody, Reduced Expression 1 Protein antibody, Reduced expression protein 1 antibody, Rex-1 antibody, REX1 antibody, REX1 transcription factor antibody, Zfp-42 antibody, Zfp42 antibody, ZFP42 zinc finger protein antibody, ZFP42_HUMAN antibody |
| hREX-1 抗体, Reduced Expression 1 Protein 抗体, Reduced expression protein 1 抗体, Rex-1 抗体, REX1 抗体, REX1 转录因子抗体, Zfp-42 抗体, Zfp42 抗体, ZFP42 锌指蛋白抗体, ZFP42_HUMAN 抗体 |
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