E Cadherin Rabbit Polyclonal Antibody(0407-25)

Cadherin-1 or Epithelial cadherin (E-cadherin), (not to be confused with the APC/C activator protein CDH1) is a protein that in humans is encoded by the CDH1 gene. Mutations are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. CDH1 has also been designated as CD324 (cluster of differentiation 324). It is a tumor suppressor gene. Cadherin-1 is a classical member of the cadherin superfamily. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail.

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概述

产品名称

E Cadherin Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within mouse E-Cadherin aa 595-644 / 882.

种属反应性

Human, Mouse

验证应用

WB, IF-Cell, FC

分子量

Predicted band size: 97 kDa

阳性对照

HCT116, SKOV-3, Hela.

偶联

unconjugated

RRID

AB_3068640

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度


  • WB

  • 1:500


  • IF-Cell

  • 1:50-1:200


  • FC

  • 1:50-1:100

靶点

功能

Cadherin-1 or Epithelial cadherin (E-cadherin), (not to be confused with the APC/C activator protein CDH1) is a protein that in humans is encoded by the CDH1 gene. Mutations are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. CDH1 has also been designated as CD324 (cluster of differentiation 324). It is a tumor suppressor gene. Cadherin-1 is a classical member of the cadherin superfamily. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells, and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function.

背景文献

1. Meigs T.E et al. Galpha12 and Galpha13 negatively regulate the adhesive functions of cadherin. J Biol Chem 277:24594-24600 (2002).

2. Agiostratidou G et al. The cytoplasmic sequence of E-cadherin promotes non-amyloidogenic degradation of A beta precursors. J Neurochem 96:1182-1188 (2006).

组织特异性

Non-neural epithelial tissues.

翻译后修饰

During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation (By similarity).; N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location.; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.; O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Thr-285 and Thr-509 are O-mannosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3.

亚细胞定位

Cell membrane.

UNIPROT #

SwissProt: P12830 Human

SwissProt: P09803 Mouse

别名

Arc 1 antibody

CADH1_HUMAN antibody

Cadherin 1 antibody

cadherin 1 type 1 E-cadherin antibody

Cadherin1 antibody

CAM 120/80 antibody

CD 324 antibody

CD324 antibody

CD324 antigen antibody

cdh1 antibody


上海睿远生物医药科技有限公司
电话:19946275258

Email: 498878287@qq.com 

官方网址:www.fulaibio.com       

E Cadherin Rabbit Polyclonal Antibody(0407-25)(图1)


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