货号： 0407-21 受体酪氨酸激酶，结合 EGF 家族配体并激活多种信号级联反应，将细胞外信号转化为适当的细胞反应。已知配体包括 EGF、TGFA/TGF-α、双调蛋白、epigen/EPGN、BTC/β-细胞素、epiregulin/EREG 和 HBEGF/肝素结合 EGF。配体结合触发受体同源或异源二聚化以及关键胞质残基的自磷酸化。磷酸化的受体招募适配蛋白（如 GRB2），进而激活复杂的下游信号级联反应。激活至少 4 种主要下游信号通路，包括 RAS-RAF-MEK-ERK、PI3 激酶-AKT、PLCγ-PKC 和 STATs 模块。还可能激活 NF-κB 信号通路。
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| 产品名称 | EGFR Rabbit Polyclonal Antibody |
| EGFR 兔多克隆抗体 | |
| 抗体类型 | Rabbit Polyclonal Antibody |
| 兔多克隆抗体 | |
| 免疫原 | Synthetic peptide within mouse EGFR aa 11-60 / 1,210 |
| 小鼠 EGFR 氨基酸 11-60 / 1,210 的合成肽 | |
| 种属反应性 | Human, Mouse, Rat |
| 人类、小鼠、大鼠 | |
| 验证应用 | WB, IF-Cell, IHC-P |
| 蛋白质印迹 (WB)、细胞免疫荧光 (IF-Cell)、免疫组化 (IHC-P) | |
| 分子量 | Predicted band size: 134 kDa |
| 预测条带大小：134 kDa | |
| 阳性对照 | A431 cell lysates, A172, rat stomach tissue, human stomach cancer tissue |
| A431 细胞裂解液、A172、大鼠胃组织、人胃癌组织 | |
| 偶联 | Unconjugated |
| 非偶联 | |
| RRID | AB_3068639 |
| AB_3068639 | |
| 产品特性 | |
| - 形态 | Liquid |
| 液体 | |
| - 浓度 | 1ug/ul |
| 1ug/ul | |
| - 存放说明 | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| 解冻后储存于 +4℃。分装后储存于 -20℃。避免反复冻融。 | |
| - 存储缓冲液 | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| 1*PBS (pH7.4), 0.2% BSA, 40% 甘油。防腐剂：0.05% 叠氮化钠。 | |
| - 亚型 | IgG |
| IgG | |
| - 纯化方式 | Immunogen affinity purified |
| 免疫原亲和纯化 | |
| 应用稀释度 | |
| - WB | 1:500-1:1,000 |
| 1:500-1:1,000 | |
| - IF-Cell | 1:50-1:200 |
| 1:50-1:200 | |
| - IHC-P | 1:50-1:200 |
| 1:50-1:200 | |
| 靶点 | |
| - 功能 | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. |
| 受体酪氨酸激酶，结合 EGF 家族配体并激活多种信号级联反应，将细胞外信号转化为适当的细胞反应。已知配体包括 EGF、TGFA/TGF-α、双调蛋白、epigen/EPGN、BTC/β-细胞素、epiregulin/EREG 和 HBEGF/肝素结合 EGF。配体结合触发受体同源或异源二聚化以及关键胞质残基的自磷酸化。磷酸化的受体招募适配蛋白（如 GRB2），进而激活复杂的下游信号级联反应。激活至少 4 种主要下游信号通路，包括 RAS-RAF-MEK-ERK、PI3 激酶-AKT、PLCγ-PKC 和 STATs 模块。还可能激活 NF-κB 信号通路。 | |
| 背景文献 | 1. Lupberger J. et al. Nat. Med. 17:589-595 (2011) 2. Groenestege W.M.T. et al. J. Clin. Invest. 117:2260-2267 (2007) |
| 1. Lupberger J. 等. Nat. Med. 17:589-595 (2011) 2. Groenestege W.M.T. 等. J. Clin. Invest. 117:2260-2267 (2007) | |
| 序列相似性 | Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. |
| 属于蛋白激酶超家族，Tyr 蛋白激酶家族，EGF 受体亚家族。 | |
| 组织特异性 | Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. |
| 广泛表达。亚型 2 也在卵巢癌中表达。 | |
| 翻译后修饰 | Phosphorylated on Tyr residues in response to EGF. Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.; Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity).; Palmitoylated on Cys residues by ZDHHC20. Palmitoylation inhibits internalization after ligand binding, and increases the persistence of tyrosine-phosphorylated EGFR at the cell membrane. Palmitoylation increases the amplitude and duration of EGFR signaling.; Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. |
| 在 EGF 刺激下 Tyr 残基磷酸化。Ser-695 的磷酸化是部分的，仅在 Thr-693 磷酸化时发生。PRKD1 对 Thr-678 和 Thr-693 的磷酸化抑制 EGF 诱导的 MAPK8/JNK1 激活。PTPRJ 的去磷酸化阻止内吞并稳定受体在质膜上的定位。Arg-1199 的甲基化刺激 Tyr-1197 的自磷酸化，并增强与 PTPN6 的相互作用。Tyr-1092 和/或 Tyr-1110 的自磷酸化招募 STAT3。PTPN1 和 PTPN2 去磷酸化；EGF 刺激下单泛素化和多泛素化，不影响酪氨酸激酶活性或信号传导能力，但可能在溶酶体靶向中起作用。多泛素化主要通过 'Lys-63' 连接，但也通过 'Lys-48'、'Lys-11' 和 'Lys-29' 连接。OTUD7B 的去泛素化阻止降解。RNF115 和 RNF126 泛素化（相似性）；ZDHHC20 对 Cys 残基进行棕榈酰化。棕榈酰化抑制配体结合后的内吞，并增加酪氨酸磷酸化 EGFR 在细胞膜上的持久性。棕榈酰化增加 EGFR 信号传导的幅度和持续时间；甲基化。PRMT5 对 Arg-1199 的甲基化刺激 Tyr-1197 的磷酸化。 | |
| 亚细胞定位 | Plasma membrane, Nucleus, Endosome, Endoplasmic reticulum |
| 质膜、细胞核、内体、内质网 | |
| UNIPROT # | SwissProt: P00533 Human SwissProt: Q01279 Mouse Entrez Gene: 24329 Rat |
| SwissProt: P00533 人类 SwissProt: Q01279 小鼠 Entrez Gene: 24329 大鼠 | |
| 别名 | Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody, Cell growth inhibiting protein 40 antibody, Cell proliferation inducing protein 61 antibody, EGF R antibody, EGFR antibody, EGFR_HUMAN antibody, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody, Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody, Epidermal growth factor receptor antibody, erb-b2 receptor tyrosine kinase 1 antibody |
| Avian erythroblastic leukemia viral (v erb b) oncogene homolog 抗体, Cell growth inhibiting protein 40 抗体, Cell proliferation inducing protein 61 抗体, EGF R 抗体, EGFR 抗体, EGFR_HUMAN 抗体, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) 抗体, Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) 抗体, Epidermal growth factor receptor 抗体, erb-b2 receptor tyrosine kinase 1 抗体 |
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