Name: Cytokeratin 18 Rabbit Polyclonal Antibody(0407-1)
Brand: 上海睿远生物医药科技有限公司
Price: 询价 CNY

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产品名称	Cytokeratin 18 Rabbit Polyclonal Antibody	Cytokeratin 18 兔多克隆抗体
抗体类型	Rabbit Polyclonal Antibody	兔多克隆抗体
免疫原	Synthetic peptide within mouse Cytokeratin 18 aa 374-423 / 423	小鼠 Cytokeratin 18 氨基酸 374-423 / 423 合成肽
种属反应性	Human, Mouse, Rat	人、小鼠、大鼠
验证应用	WB, IF-Cell, IHC-P	蛋白质印迹、细胞免疫荧光、免疫组化
分子量	Predicted band size: 48 kDa	预测条带大小：48 kDa
阳性对照	Mouse liver tissue lysate, A431 cell lysates, 293T, A549, SW480, mouse kidney tissue, human liver tissue, human liver carcinoma tissue, mouse lung tissue	小鼠肝组织裂解液、A431 细胞裂解液、293T、A549、SW480、小鼠肾组织、人肝组织、人肝癌组织、小鼠肺组织
偶联	Unconjugated	非偶联
RRID	AB_3068636	AB_3068636
产品特性	Product Characteristics	产品特性
形态	Liquid	液体
浓度	1ug/ul	1微克/微升
存放说明	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles	解冻后存放于 +4℃。分装后存放于 -20℃。避免反复冻融。
存储缓冲液	1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide	1*PBS (pH7.4), 0.2% BSA, 25% 甘油。防腐剂：0.05% 叠氮化钠。
亚型	IgG	IgG
纯化方式	Immunogen affinity purified	免疫原亲和纯化
应用稀释度	Application Dilution	应用稀释度
WB	1:500-1,000	1:500-1,000
IF-Cell	1:200	1:200
IHC-P	1:100-1:400	1:100-1:400
靶点	Target	靶点
功能	Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.	Keratin 18 是一种 I 型细胞角蛋白。它与它的纤维伴侣 keratin 8 一起，可能是中间纤维基因家族中最常见的产物。它们在身体的单层上皮组织中表达。该基因的突变与隐源性肝硬化有关。已发现该基因有两种编码相同蛋白的转录变体。Keratin 18 通常与 keratin 8 和 keratin 19 一起用于区分上皮来源的细胞和造血细胞，以检测血液中的循环肿瘤细胞。
背景文献	Background Literature	背景文献
1	Ku N O et al. Identification of the major physiologic phosphorylation site of human keratin 18: potential kinases and a role in filament reorganization. J Cell Biol 127:161-171 (1994)	Ku N O 等。人 keratin 18 主要生理磷酸化位点的鉴定：潜在激酶及其在纤维重组中的作用。细胞生物学杂志 127:161-171 (1994)
2	Ku N.O et al. Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant. J Cell Biol 131:1303-1314 (1995)	Ku N.O 等。表达 keratin 18 保守精氨酸突变体的转基因小鼠中的慢性肝炎、肝细胞脆性和可溶性磷酸糖角蛋白增加。细胞生物学杂志 131:1303-1314 (1995)
序列相似性	Sequence Similarity	序列相似性
	Belongs to the intermediate filament family	属于中间纤维家族
组织特异性	Tissue Specificity	组织特异性
	Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma	在结肠、胎盘、肝脏中表达，在宫颈外膜中表达较弱。在乳腺癌淋巴结中观察到表达增加。
翻译后修饰	Post-translational Modifications	翻译后修饰
	Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation	Ser-34 的磷酸化在有丝分裂期间增加。在肝硬化肝脏中 Ser-53 过度磷酸化。IL-6 增加磷酸化。在上皮细胞凋亡期间被 caspase 蛋白水解切割。切割发生在 Asp-238，由 caspase-3、caspase-6 或 caspase-7 介导。O-糖基化增加溶解度，并通过诱导蛋白酶体降解降低稳定性。
亚细胞定位	Subcellular Location	亚细胞定位
	Cytoplasm, perinuclear region	细胞质、核周区域
UNIPROT #	UNIPROT #	UNIPROT #
	SwissProt: P05783 Human	SwissProt: P05783 人
	SwissProt: P05784 Mouse	SwissProt: P05784 小鼠
	SwissProt: Q5BJY9 Rat	SwissProt: Q5BJY9 大鼠
别名	Aliases	别名
	Cell proliferation inducing gene 46 protein antibody	细胞增殖诱导基因 46 蛋白抗体
	Cell proliferation inducing protein 46 antibody	细胞增殖诱导蛋白 46 抗体
	Cell proliferation-inducing gene 46 protein antibody	细胞增殖诱导基因 46 蛋白抗体
	CK 18 antibody	CK 18 抗体
	CK-18 antibody	CK-18 抗体
	CK18 antibody	CK18 抗体
	CYK 18 antibody	CYK 18 抗体
	CYK18 antibody	CYK18 抗体
	Cytokeratin 18 antibody	Cytokeratin 18 抗体
	Cytokeratin endo B antibody	Cytokeratin endo B 抗体