Name: c-kit Rabbit Polyclonal Antibody（0108-4）
Brand: 上海睿远生物医药科技有限公司
Price: 询价 CNY

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概述	Overview	概述
产品名称	c-kit Rabbit Polyclonal Antibody	c-kit 兔多克隆抗体
抗体类型	Rabbit Polyclonal Antibody	兔多克隆抗体
免疫原	Synthetic peptide within Human c-kit aa 109-150.	人源 c-kit 氨基酸 109-150 的合成肽
种属反应性	Human	人
验证应用	WB, IF-Cell	蛋白质印迹（WB），细胞免疫荧光（IF-Cell）
分子量	Predicted band size: 110 kDa	预测条带大小：110 kDa
阳性对照	Jurkat	Jurkat 细胞
偶联	Unconjugated	非偶联
RRID	AB_3068633	AB_3068633
产品特性	Product Characteristics	产品特性
形态	Liquid	液体
浓度	1ug/ul	1微克/微升
存放说明	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.	解冻后存放于 +4℃。分装后存放于 -20℃ 或 -80℃。避免反复冻融。
存储缓冲液	1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.	1*PBS (pH7.4), 0.2% BSA, 40% 甘油。防腐剂：0.05% 叠氮化钠。
亚型	IgG	IgG
纯化方式	Immunogen affinity purified.	免疫原亲和纯化
应用稀释度	Application Dilution	应用稀释度
WB	1:500	1:500
IF-Cell	1:100	1:100
靶点	Target	靶点
功能	CD117 Antigen, also called KIT or C-kit receptor, is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.	CD117 抗原，也称为 KIT 或 C-kit 受体，是一种在造血干细胞及其他细胞类型表面表达的细胞因子受体。它具有酪氨酸蛋白激酶活性。配体结合导致 KIT 的自磷酸化及其与底物（如磷脂酰肌醇 3-激酶，Pi3K）的结合。该基因的突变与胃肠道间质瘤、肥大细胞疾病、急性髓性白血病和白斑病相关。
背景文献	Background Literature	背景文献
1	Yarden, Y., et al. (1987) EMBO (Eur. Mol. Biot. Organ.) J. 6:3341-3351.	Yarden, Y., 等. (1987) EMBO (欧洲分子生物学组织) 杂志 6:3341-3351.
2	Qiu, F. H.,et al. (1988) EMBO (Eur. Mol. Biot. Organ.) J. 7:1003-1011.	Qiu, F. H., 等. (1988) EMBO (欧洲分子生物学组织) 杂志 7:1003-1011.
3	Besmer, et al. 1986. Nature (Lond.). 320:415-421.	Besmer, 等. 1986. 自然 (伦敦). 320:415-421.
4	Zsebo, K. M., et al. (1990) Cell. 63:195-201.	Zsebo, K. M., 等. (1990) 细胞. 63:195-201.
5	Spritz RA, Giebel LB, Holmes SA. (1992) Am J Hum Genet. Feb;5 (2):261-9.	Spritz RA, Giebel LB, Holmes SA. (1992) 美国人类遗传学杂志. 2月;5 (2):261-9.
序列相似性	Sequence Similarity	序列相似性
	Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.	属于蛋白激酶超家族。Tyr 蛋白激酶家族。CSF-1/PDGF 受体亚家族。
组织特异性	Tissue Specificity	组织特异性
	Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.	异构体 1 和异构体 2 在精原细胞和 Leydig 细胞中检测到。异构体 3 在圆形精子细胞、伸长精子细胞和精子中检测到（蛋白质水平）。广泛表达。在造血系统、胃肠道系统、黑色素细胞和生殖细胞中检测到。
翻译后修饰	Post-translational Modifications	翻译后修饰
	Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.; Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.	由 SOCS6 泛素化。KIT 在 KITLG/SCF 结合诱导的自磷酸化后迅速泛素化，导致内化和降解。；在酪氨酸残基上自磷酸化。KITLG/SCF 结合增强自磷酸化。异构体 1 在没有添加 KITLG/SCF 的情况下显示出低水平的酪氨酸磷酸化（体外）。激酶活性通过蛋白激酶 C 家族成员在丝氨酸残基上的磷酸化下调。Tyr-568 的磷酸化是与 PTPN11/SHP-2、CRK（异构体 Crk-II）和 SRC 酪氨酸蛋白激酶家族成员相互作用所必需的。Tyr-570 的磷酸化是与 PTPN6/SHP-1 相互作用所必需的。Tyr-703、Tyr-823 和 Tyr-936 的磷酸化对于与 GRB2 的相互作用很重要。Tyr-721 的磷酸化对于与 PIK3R1 的相互作用很重要。Tyr-823 和 Tyr-936 的磷酸化对于与 GRB7 的相互作用很重要。
亚细胞定位	Subcellular Location	亚细胞定位
	Cytoplasm, membrane	细胞质，膜
UNIPROT #	SwissProt: P10721 Human	SwissProt: P10721 人
别名	C Kit antibody, c-Kit antibody, c-Kit Ligand antibody, CD117 antibody, Kit antibody, Kit Ligand antibody, KIT oncogene antibody, KIT proto oncogene receptor tyrosine kinase antibody, KIT_HUMAN antibody, Mast cell growth factor receptor antibody	C Kit 抗体, c-Kit 抗体, c-Kit 配体抗体, CD117 抗体, Kit 抗体, Kit 配体抗体, KIT 癌基因抗体, KIT 原癌基因受体酪氨酸激酶抗体, KIT_HUMAN 抗体, 肥大细胞生长因子受体抗体