c-kit Rabbit Polyclonal Antibody（0108-4）

功能

CD117 Antigen, also called KIT or C-kit receptor, is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.

背景文献

1. Yarden, Y., et al. (1987) EMBO (Eur. Mol. Biot. Organ.) J. 6:3341-3351.

2. Qiu, F. H.,et al. (1988) EMBO (Eur. Mol. Biot. Organ.) J. 7:1003-1011.

3. Besmer, et al. 1986. Nature (Lond.). 320:415-421.

4. Zsebo, K. M., et al. (1990) Cell. 63:195-201.

5. Spritz RA, Giebel LB, Holmes SA. (1992) Am J Hum Genet. Feb;5 (2):261-9.

序列相似性

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

组织特异性

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

翻译后修饰

Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.; Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.

亚细胞定位

Cytoplasm, membrane

UNIPROT #

SwissProt: P10721 Human

别名